MAB3034
Anti-DNA Antibody, single stranded, clone 16-19
clone 16-19, Chemicon®, from mouse
Synonym(e):
Single-Stranded DNA Antibody
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
16-19, monoclonal
Speziesreaktivität (Voraussage durch Homologie)
all
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
Isotyp
IgG2aκ
Versandbedingung
wet ice
Posttranslationale Modifikation Target
unmodified
Spezifität
Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA.
ANTIGEN BINDING PROFILE:
Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53
a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
BINDING ASSAY
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ISOELECTRIC POINT = 8.3
ANTIGEN BINDING PROFILE:
Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53
a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 μg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
BINDING ASSAY
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ISOELECTRIC POINT = 8.3
Immunogen
Epitope: single stranded DNA
Anwendung
Research Category
Apoptose & Krebs
Apoptose & Krebs
Research Sub Category
Apoptose - Weiterführende Produkte
Zellzyklus, DNA-Replikation & -Reparatur
Apoptose - Weiterführende Produkte
Zellzyklus, DNA-Replikation & -Reparatur
ELISA
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.
Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA.
Physikalische Form
0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide.
Format: Purified
Lagerung und Haltbarkeit
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sie haben nicht das passende Produkt gefunden?
Probieren Sie unser Produkt-Auswahlhilfe. aus.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart.
Ciccia, A; Bredemeyer, AL; Sowa, ME; Terret, ME; Jallepalli, PV; Harper, JW; Elledge, SJ
Genes & Development null
E2F1 localizes to sites of UV-induced DNA damage to enhance nucleotide excision repair.
Guo, R; Chen, J; Zhu, F; Biswas, AK; Berton, TR; Mitchell, DL; Johnson, DG
The Journal of Biological Chemistry null
DNA replication is altered in Immunodeficiency Centromeric instability Facial anomalies (ICF) cells carrying DNMT3B mutations.
Lana, E; Megarbane, A; Tourriere, H; Sarda, P; Lefranc, G; Claustres, M; De Sario, A
European Journal of Human Genetics null
Evidence of DNA transfer through F-pilus channels during Escherichia coli conjugation.
An-Chi Shu,Chien-Chen Wu,Yi-Yang Chen,Hwei-Ling Peng,Hwan-You Chang,Tri-Rung Yew
Langmuir null
In Xenopus egg extracts, DNA replication initiates preferentially at or near asymmetric AT sequences.
Stanojcic, S; Lemaitre, JM; Brodolin, K; Danis, E; Mechali, M
Molecular and cellular biology null
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.