MAB3484

Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4

clone L12B4, Chemicon^®, from mouse

• Synonym(e): CFTR
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: L12B4, monoclonal
• Speziesreaktivität: human
• Darf nicht reagieren mit: shark, mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2a
• NCBI-Hinterlegungsnummer: NM_000492.3
• UniProt-Hinterlegungsnummer: P13569
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CFTR(1080)

Beschreibung

Spezifität

Human cystic fibrosis transmembrane conductance regulator (CFTR). Recognizes an epitope at the cytoplasmic region preceding the first nucleotide binding domain between residues 386 to 412.

Recognizes CFTR, Mr 170kDa and two additional proteins at ~ Mr 100 and 140kDa.

Immunogen

Epitope: a.a. 386-412 of human CFTR

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Ionenkanäle & -transporter

Anti-Cystic Fibrosis Transmembrane Conductance Regulator Antibody, a.a. 386-412, clone L12B4 is an antibody against Cystic Fibrosis Transmembrane Conductance Regulator for use in IC, IP & WB.

Western blot: 1-10μg/mL, note do not boil the lysate, incubate at 80C for 30 minutes prior to running SDS-PAGE. CTFR aggregates upon boiling. Antibody recognizes CFTR at 170kDa and two additional proteins at 100 & 140kDa.

Immunoprecipitation

Immunofluorescence

Note: Does not work on paraffin embedded tissue.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Format: Purified

Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots up to 6 months.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable