MAB1810
Anti-ADAMTS-1 Antibody, clone 5D4E11B6
serum, clone 5D4E11B6, Chemicon®
Synonym(e):
METH-1
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Alle Fotos(3)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
serum
Antikörper-Produkttyp
primary antibodies
Klon
5D4E11B6, monoclonal
Speziesreaktivität
human, mouse, rat
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ADAMTS1(9510)
Allgemeine Beschreibung
ADAMTS-1 is a member of a family of extracellular proteases known as ADAMTS (a disintegrin and metalloprotease with thrombospondin motifs). Members of the ADAMTS family share distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TSP) motif. Individual members of this family differ in the number of C-terminal TSP motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TSP motifs. ADAMTS-1 cleaves aggrecan, a cartilage proteoglycan and appears to demonstrate angiogenesis inhibitory activity. The maturation of ADAMTS-1 requires two independent and sequential processing events that may release two forms of the protein. With the cleavage of the prodomain from the 110 kDa zymogen, an 87 kDa active form remains which can be further processed by removal of the catalytic subunit from the TSP repeats, leaving the C-termianl 65 kDa soluble form. The proteolytic deletion of the last two TSP repeats, is probably significant for the in vivo function of this protein.
Spezifität
Clone 5D4E11B6 recognizes human ADAMTS-1.
Reactivity with other species has not been tested.
Immunogen
Myc-His carboxyl tagged full-length recombinant protein based on the human sequence.
Anwendung
Detect ADAMTS-1 using this Anti-ADAMTS-1 Antibody, clone 5D4E11B6 validated for use in ELISA, IP & WB.
Western blot: 1:2000 - 1:4000 dilution on A431 cell lysate
Note: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms.
Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate
Note: MW bands detected vary by cell line or sample with detection of zymogen and/or active forms.
Immunoprecipitation: confirm by immunoblot using clone 5D4E11B6; 1:50 using clone 5C6D5 (Catalog # MAB1771) to immunoprecipitate ADAMTS-1 from 500 µg of HEK293 whole cell lysate or PC-12 whole cell lysate
Zielbeschreibung
110 / 87 / 65 kDa
Physikalische Form
Ascites. Liquid containing 0.05% NaN3.
Hinweis zur Analyse
Control
A431 cell lysate
HEK293 cell lysate
PC-12 cell lysate
Embryonic mouse brain lysate
MCF-7 cell lysate
A431 cell lysate
HEK293 cell lysate
PC-12 cell lysate
Embryonic mouse brain lysate
MCF-7 cell lysate
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
nwg
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Alfonso Luque et al.
The Journal of biological chemistry, 278(26), 23656-23665 (2003-04-30)
ADAMTS1 is a metalloprotease previously shown to inhibit angiogenesis in a variety of in vitro and in vivo assays. In the present study, we demonstrate that ADAMTS1 significantly blocks VEGFR2 phosphorylation with consequent suppression of endothelial cell proliferation. The effect
J C Rodriguez-Manzaneque et al.
The Journal of biological chemistry, 275(43), 33471-33479 (2000-08-17)
METH-1/ADAMTS1 is a member of a newly described family of genes that contain metalloprotease, disintegrin, and thrombospondin-like motifs. We have recently shown that METH-1 protein is a potent inhibitor of angiogenesis. Here, we demonstrate that secreted human pro-METH-1 is processed
Yunjie Qiu et al.
Frontiers in aging neuroscience, 14, 896522-896522 (2022-08-27)
Amyloid-β (Aβ) derived from amyloid precursor protein (APP) hydrolysis is acknowledged as the predominant hallmark of Alzheimer's disease (AD) that especially correlates to genetics and daily activities. In 2019, meta-analysis of AD has discovered five new risk loci among which
Maíra de Assis Lima et al.
Cellular signalling, 77, 109827-109827 (2020-11-09)
ADAMTSs (A Disintegrin And Metalloproteinase with ThromboSpondin motifs) are secreted proteases dependent on Zn2+/Ca2+, involved in physiological and pathological processes and are part of the extracellular matrix (ECM). Here, we investigated if ADAMTS-1 is required for invasion and migration of
Suély V Silva et al.
PloS one, 11(10), e0165061-e0165061 (2016-10-21)
Proteins secreted in the extracellular matrix microenvironment (ECM) by tumor cells are involved in cell adhesion, motility, intercellular communication and invasion. The tumor microenvironment is expansively modified and remodeled by proteases, resulting in important changes in both cell-cell and cell-ECM
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