ABE1361

Anti-NuMA1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Nuclear mitotic apparatus protein 1, NuMA protein, SP-H antigen
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001273490
• UniProt-Hinterlegungsnummer: Q14980
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NUMA1(4926)

Beschreibung

Allgemeine Beschreibung

NuMA1 or NuMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NuMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NuMA1 resides in the nuclear matrix during interphase. NuMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NuMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis.

Spezifität

Recognizes NuMA1

Immunogen

Three different peptides corresponding to multiple locations of NuMA1 isoform 1. Peptide, region between amino acids 1-240. Peptide 2, amino acids 1300-1500. Peptide 3, amino acids 1860-2115. Note that these peptides are also found in isoform 2.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Chromatin-Biologie (ChIP)

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot was reported to detect NuMA1 in HeLa cells.

This Anti-NuMA1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of NuMA1.

Qualität

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected NuMA1 in a A431 cell lysate.

Zielbeschreibung

~ 238 kDa observed

Physikalische Form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide.

Lagerung und Haltbarkeit

Store at -20°C

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2