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Key Documents

AB1559P

Sigma-Aldrich

Anti-Adenosine A2a Receptor Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

pig, bovine, canine, human

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ADORA2A(135)

Spezifität

Adenosine A2a receptor. Recognizes a band of approximately 45 kDa in human (coronary artery), and rat, porcine and bovine striatal membranes.

Immunogen

Amino acid sequence near the 4th C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected is not present in A1, A2b and A3 receptors. Control peptide is available for this product (Catalog Number AG285).

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Neurotransmitter & Rezeptoren

Tagesrhythmus & Schlaf
Anti-Adenosine A2a Receptor Antibody is an antibody against Adenosine A2a Receptor for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen tissue sections

Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.

Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.

ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)

Optimal working dilutions must be determined by end user.

Physikalische Form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA and 0.05% sodium azide.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

A L Tucker et al.
Cardiovascular research, 27(1), 62-67 (1993-01-01)
Four subtypes of adenosine receptors have recently been cloned from thyroid, brain and testis. In this review we have summarised properties of these purinergic receptors. The cloned A1 and A2 subtypes are probably similar or identical to receptors that exist
R B Marala et al.
The Journal of pharmacology and experimental therapeutics, 286(2), 1051-1057 (1998-08-08)
Antipeptide antibody was raised in rabbit against the sequence (361-390) of RDC-8, the presumed adenosine A2A receptor cDNA from canine. The antibody titer was estimated by solid phase radioimmunoassay. Western blot analysis under reducing conditions identified a major 45 +/-
Expression of adenosine receptors in the preglomerular microcirculation.
Edwin K Jackson, Chongxue Zhu, Stevan P Tofovic
American Journal of Physiology: Renal Physiology null

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