553504

RAD51, His•Tag^®, Pneumocystis carinii, Recombinant, E. coli

• UNSPSC-Code: 12352202
• NACRES: NA.42

Eigenschaften

• Assay: ≥95% (SDS-PAGE)
• Qualitätsniveau: 100
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis carinii RAD51 is equivalent to bacterial RecA and the homologue of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.

Recombinant, Pneumocystis carinii RAD51 fused at the N-terminus to a His•Tag sequence and expressed in E. coli. The Pneumocystis cariniiRAD51 is equivalent to bacterial RecA and the homolog of yeast RAD51. It plays a pivotal role in the homologous recombination pathway for DNA double-strand break (DSB) repair. RAD51 has been shown to colocalize with tumor suppressor proteins BRCA1 (Cat. No. 203659) and BRCA2 in DNA-damage-induced foci and RAD51 is known to interact directly with BRCA2.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit equals 1 ng of purified protein. 20 units are sufficient for a DNA-protein assay and 100 units are sufficient for a protein-protein interaction assay.

Physikalische Form

In 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.

Sonstige Hinweise

West, S.C. 2003. Nat. Rev. Mol. Cell Biol.4, 435.
Jasin, M. 2002. Oncogene21, 8981.
Symington, L.S., et al. 2002. Microbiol. Mol. Biol. Rev.66, 630.
Daboussi, F., et al. 2002. Cell. Signal.14, 969.
Liu, Y. and West, S.C. 2002. Breast Cancer Res.4, 9.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable