492899

Nuclease, Staphylococcus aureus

Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups.

• Synonym(e): Nuclease, Staphylococcus aureus
• CAS-Nummer: 9013-53-0
• EC-Nummer: 3.1.31.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131731
• UNSPSC-Code: 12352202
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Staphylococcus aureus
• Qualitätsniveau: 100
• Form: lyophilized solid
• Spezifische Aktivität: ≥15,000 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; desiccated (hygroscopic)
• Farbe: off-white
• Löslichkeit: aqueous buffer: 1 mg/mL
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.

Sonstige Hinweise

Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable