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Key Documents

487923

Sigma-Aldrich

Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3)

liquid, clone CC22.8C7.3, Calbiochem®

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

CC22.8C7.3, monoclonal

Form

liquid

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität (Voraussage durch Homologie)

all

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Isotyp

IgG2b

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... NOS1(4842)

Allgemeine Beschreibung

Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.
Recognizes nitrotyrosine.
This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.

Immunogen

peroxynitrite-treated KLH

Anwendung

Immunoblotting (2 µg/ml)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.

Rekonstituierung

Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Hinweis zur Analyse

Positive Control
Mouse macrophages treated with peroxynitrite

Sonstige Hinweise

Myatt, L., et al. 1996. Hypertension28, 488.
Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.
Variables associated with assay conditions will dictate the proper working dilution.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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L Myatt et al.
Hypertension (Dallas, Tex. : 1979), 28(3), 488-493 (1996-09-01)
The interaction of nitric oxide and superoxide produces peroxynitrite anion, a strong, long-lived oxidant with pronounced deleterious effects that may cause vascular damage. The formation and action of peroxynitrite can be detected by immunohistochemical localization of nitrotyrosine residues. We compared
Teresa Tiago et al.
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