374825

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin Free, is a large, multi-subunit, O2-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

• Synonym(e): Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid
• CAS-Nummer: 9013-72-3
• MDL-Nummer: MFCD00131280
• UNSPSC-Code: 12352202
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 200
• Assay: ≥95% (size exclusion chromatography)
• Form: liquid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: do not freeze
• Verunreinigungen: ≤25 EU/mg Endotoxin; ≥1.5 x 10⁻³ copper to protein ratio; ≥5 mg/mL Protein
• Farbe: clear bluish
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. This high molecular weight glycoprotein is extracted from a marine snail species called Megathura crenulate.

Anwendung

Hemocyanin, Keyhole Limpet, Megathura crenulata, high purity, endotoxin-free, sterile-liquid has been used as an antigen to test the polyreactivity of monoclonal antibody (mAb) using enzyme-linked immunosorbent assay(ELISA). It has also been used to immunize animals to study KLH-specific ex vivo recall responses and specific T-cell dependent antibody response (TDAR) in monkeys.

Biochem./physiol. Wirkung

Keyhole limpet hemocyanin (KLH) stimulates both cell-mediated and humoral responses in animals and humans. It elicits immunostimulatory properties and also functions as a vaccine adjuvant. KLH serves as a carrier protein to produce antibodies and haptens like peptides and oligosaccharides. It is used to treat bladder cancer.

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 83 mM sodium phosphate, 150 mM NaCl, pH 7.2.

Angaben zur Herstellung

KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable