344932

Furin, Human, Recombinant

• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Form: liquid
• Qualitätsniveau: 100
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like pro-hormone convertase (PC) family. Members of this family cleave pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins. The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus.

Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Supplied as a crude preparation of soluble furin obtained from cytoplasmic extracts. Furin is a type I membrane protein that processes stromelysin-3 (MMP-11) and MT1-MMP. It cleaves pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins.

This preparation was subjected to UV irradiation for 5 min to inactivate any residual vaccinia virus.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of furin that will cleave 1.0 pmol AMC from the substrate pGlu-Arg-Thr-Lys-Arg-AMC per min at 30°C, pH 7.5.

Physikalische Form

In 20 mM Bis-Tris buffer, 1 mg/ml BSA, pH 7.0.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224.
Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326.
Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211.
Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953.
Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable