16-218

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 conjugate

clone 3H11, Upstate^®, from mouse

• Synonym(e): H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: CY5 conjugate
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 3H11, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Upstate^®
• Methode(n): immunofluorescence: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NM_003493.2
• UniProt-Hinterlegungsnummer: Q16695
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer10)
• Angaben zum Gen: human ... HIST1H3F(8968)

Beschreibung

Allgemeine Beschreibung

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Spezifität

Expected to cross react with all vertebrate species.

Histone H3 phosphorylated at Ser10

Immunogen

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3, clone 3H10. This is a Cy5 conjugate of Anti-phospho-Histone H3 (Ser10), clone 3H10, Catalog # 05-806.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Histone

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, Cy5 Conjugate is a Mouse Monoclonal Antibody for detection of Histone H3 phosphorylated at serine 10. This purified mAb, also known as H3S10p,
is labeled with Cy5.5 NHS ester (Cy5) and validated in IF, WB.

Qualität

Routinely evaluated by immunoblot in acid extrated protein from mitotic HeLa cells treated with colcemid (17-306).

Zielbeschreibung

17kDa

Physikalische Form

Protein G Purified

Lagerung und Haltbarkeit

1 year at 4°C from date of shipment

Hinweis zur Analyse

Control
Acid extracts from colcemid treated HeLa cells (lane 2)

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable