07-217-I

Anti-NAG-1/GDF15

from rabbit, purified by affinity chromatography

• Synonym(e): Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta, Placental bone morphogenetic protein, Prostate differentiation factor
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI-Hinterlegungsnummer: NP_004855
• UniProt-Hinterlegungsnummer: Q99988
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Growth/differentiation factor 15 (UniProt: Q99988; also known as GDF-15, Macrophage inhibitory cytokine 1, MIC-1, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1, Placental TGF-beta, Placental bone morphogenetic protein, and Prostate differentiation factor) is encoded by the GDF15 (also known as MIC1, PDF, PLAB, PTGFB) gene (Gene ID: 9518) in human. NAG-1 is a member of the TGF-beta super family, however, it does not display strong homology to existing families. NAG-1 is synthesized as a 308-amino acid pro-NAG-1 monomer and then dimerizes by a specific disulfide linkage. The pro-NAG-1 dimer is then cleaved by furin-like proteases at an RXXR site, forming a 112 amino acid C-terminal dimeric protein and pro-peptide. It is expressed in placenta and is found in lower levels in prostate, kidney, and colon. Its expression is significantly increased during inflammation, injury, and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. NAG-1 expression is also shown to increase following exposure to a number of non-steroidal anti-inflammatory drugs. Induced expression of NAG-1 is shown to result in stimulation of apoptosis and inhibition of cell growth.

Spezifität

This polyclonal antibody specifically detects Growth/differentiation factor 15 in human.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal end of human NAG-1/GDF15.

Anwendung

Research Category
Zelluläre Signaltransduktion

Detect Growth/differentiation factor 15 using this rabbit polyclonal Anti-NAG-1/GDF15, Cat. No. 07-217-I, useing Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: 2 µg/mL from a representative lot detected NAG-1/GDF15 in 10 µg of HepG2 conditioned media.



Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NAG-1/GDF15 in human placenta tissue.

Qualität

Evaluated by Western Blotting in LNCap Conditioned Media.

Western Blotting Analysis: 2 µg/mL of this antibody detected NAG-1/GDF15 in 10 µg of LNCap Conditioned Media.

Zielbeschreibung

34.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Verlinkung

Replaces: 07-217

Physikalische Form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1