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Key Documents

05-592

Sigma-Aldrich

Anti-PP2A Antibody, B subunit, clone 2G9

ascites fluid, clone 2G9, Upstate®

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

2G9, monoclonal

Speziesreaktivität

rat, bovine, mouse, Xenopus, rabbit, pig, human

Hersteller/Markenname

Upstate®

Methode(n)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Angaben zum Gen

human ... PPP2CA(5515)

Spezifität

PP2A B subunit, isoform reactivity is α > δ,β > γ

Immunogen

Peptide corresponding to residues 398-411 of human PP2A, B subunit

Anwendung

Research Category
Zelluläre Signaltransduktion

Neurowissenschaft
Research Sub Category
Kinasen & Phosphatasen

Neurodegenerative Krankheiten
Detect PP2A using this Anti-PP2A Antibody, B subunit, clone 2G9 validated for use in IH, IP & WB.

Qualität

routinely evaluated by immunoblot on RIPA lysates from non-stimulated A431 cells or mouse 3T3/A31 cells

Zielbeschreibung

55kDa

Physikalische Form

Ascites
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and glycerol to 30%

Lagerung und Haltbarkeit

2 years at -20°C

Hinweis zur Analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Protein phosphatase 2A regulates estrogen receptor alpha (ER) expression through modulation of ER mRNA stability.
Keen, JC; Zhou, Q; Park, BH; Pettit, C; Mack, KM; Blair, B; Brenner, K; Davidson, NE
The Journal of Biological Chemistry null
Protein phosphatase 2A subunit assembly: the catalytic subunit carboxy terminus is important for binding cellular B subunit but not polyomavirus middle tumor antigen.
Ogris, E, et al.
Oncogene, 15, 911-917 (1997)
Jennifer B Jackson et al.
Neoplasia (New York, N.Y.), 14(7), 585-599 (2012-08-21)
Heterotrimeric protein phosphatase 2A (PP2A) consists of catalytic C (PP2Ac), structural A, and regulatory B-type subunits, and its dysfunction has been linked to cancer. Reversible methylation of PP2Ac by leucine carboxyl methyltransferase 1 (LCMT-1) and protein phosphatase methylesterase 1 (PME-1)
Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
Williams, BC; Filter, JJ; Blake-Hodek, KA; Wadzinski, BE; Fuda, NJ; Shalloway, D; Goldberg, ML
eLife null
Lihua Y Marmorstein et al.
The Journal of biological chemistry, 277(34), 30591-30597 (2002-06-12)
Bestrophin is a 68-kDa basolateral plasma membrane protein expressed in retinal pigment epithelial cells (RPE). It is encoded by the VMD2 gene, which is mutated in Best macular dystrophy, a disease characterized by a depressed light peak in the electrooculogram.

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