07-2273

Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

from rabbit, purified by affinity chromatography

• Synonym(e): DCHT, Serine/threonine-protein kinase 39
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse, human
• Speziesreaktivität (Voraussage durch Homologie): canine (based on 100% sequence homology), rat (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_037365
• UniProt-Hinterlegungsnummer: Q9UEW8
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer373)
• Angaben zum Gen: human ... STK39(27347)

Beschreibung

Allgemeine Beschreibung

As members of the (sterile 20)-like (STE20) kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with a unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.

Spezifität

This antibody recognizes SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.

Immunogen

Epitope: SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.

KLH-conjugated linear peptide corresponding to human SPAK and OSR1 phosphorylated at Ser375 and Ser325, respectively.

Anwendung

Research Category
Zelluläre Signaltransduktion

Entzündung & Immunologie

Research Sub Category
Kinasen & Phosphatasen

Oxidativer Stress

This phospho-SPAK antibody is validated for use in WB for the detection of the phospho-SPAK protein.

Qualität

Evaluated by Western Blot in hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.

Western Blot Anlaysis: 0.5 µg/mL of this antibody detected SPAK / OSR1 in 10 µg hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.

Zielbeschreibung

~60 kDa observed

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable