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GE17-0150-01

Sepharose™ CL-4B

Cytiva 17-0150-01, pack of 1 L

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About This Item

CAS Number:
61970-08-9
MDL number:
MFCD00148279
UNSPSC Code:
41106500
NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

autoclavable Autoclavable, 20 min at 120°C in pH 7

packaging

pack of 1 L

manufacturer/tradename

Cytiva 17-0150-01

matrix

4% agarose

particle size

45-165 μm

cleaning in place

2-14

working range

3-13

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General description

Sepharose™ CL-4B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose™ is available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B respectively, Both Sepharose™ and Sepharose™ CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.

Sepharose™ CL gels are cross-linked derivatives of Sepharose™ 2B, Sepharose™ 4B and Sepharose™ 6B. The cross-linked form of Sepharose™ is chemically and physically more resistant than Sepharose™ itself, offering the same selectivity with better flow characteristics. Cross-linked Sepharose™ gels are resistant to organic solvents and are thus the choice for separations in organic solvents.

Features and Benefits

  • 4% cross-linked agarose gel filtration media.
  • Proven base matrix for coupling affinity ligands
  • Higher degree of crosslinking improve matrix stability

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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