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Key Documents

91414

Sigma-Aldrich

Tris buffered saline with Tween® 20

BioUltra, tablet (for 500 mL), pH 7.6

Synonym(s):

TBST

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About This Item

UNSPSC Code:
41105319

product line

BioUltra

Quality Level

form

tablet (for 500 mL)

pH

7.6

solubility

water: 2 tablet/liter, clear, colorless

application(s)

diagnostic assay manufacturing

Related Categories

Application

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris buffered saline with Tween 20, pH 7.6 is used as a washing buffer in assays such as enzyme-linked immunosorbent assays.

Reconstitution

1 tablet dissolved in 500 mL of deionized water yields:
0.15 M sodium chloride, 0.050 m TRIS-HCl buffer; 0..05% Tween® 20;
pH 7.6 at 25°C

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Friederike Traunmüller et al.
Journal of immunoassay & immunochemistry, 26(3), 179-188 (2005-07-14)
Immunogenic glycolipids from the cell wall of Mycobacterium tuberculosis are potential capture antigens in enzyme-linked immunosorbent assays (ELISAs) for the serodiagnostis of tuberculosis. Typically, washing steps in ELISAs are performed with buffers containing a detergent. However, Tween-20, the most commonly
T Akimoto et al.
Lupus, 8(9), 761-766 (1999-12-22)
Autoantibodies against prothrombin, including lupus anticoagulant antibodies (LAC), have been identified in patients with systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS). To identify the epitopes of LAC in patients with SLE and APS, we analyzed B cell epitopes of
Lopa M Das et al.
Autophagy, 15(5), 813-826 (2019-01-22)
Cutaneous inflammation from UV radiation exposure causes epidermal damage, cellular infiltration, and secretion of pro-inflammatory mediators that exacerbate tissue destruction. Recovery is mediated chiefly by anti-inflammatory M2 macrophages that suppress inflammation and augment epidermal regeneration. Vitamin D enables anti-inflammation to
Cesare Orlandi et al.
PloS one, 6(9), e25350-e25350 (2011-10-04)
Ionotropic glutamate α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors are the major mediators of fast synaptic neurotransmission. In this work, we used primary cortical cultures from rats as a model system to study AMPA receptor regulation during in vitro cell maturation and
Bernd Zetsche et al.
Cell, 163(3), 759-771 (2015-10-01)
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here

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