AB3269P
Anti-Adiponectin Antibody
Chemicon®, from rabbit
Synonym(s):
Acrp30, AdipoQ, apM1, GBP28
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ADIPOQ(9370)
Specificity
Recognizes mouse adiponectin. Recognizes both natural and recombinant protein.
SPECIES REACTIVITIES:
There is weak reactivity with rat. Reactivity with other species has not been confirmed.
SPECIES REACTIVITIES:
There is weak reactivity with rat. Reactivity with other species has not been confirmed.
Immunogen
Recombinant mouse adiponectin from E. coli.
Application
Anti-Adiponectin Antibody detects level of Adiponectin & has been published & validated for use in ELISA & WB.
Research Category
Metabolism
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation
Lipid Metabolism & Weight Regulation
Western blot: 1:5,000-1:10,000 using ECL. Reacts with the ~35 kDa adiponectin protein. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 with 5% skim milk. Suggested incubation time is 1 hour at room temperature.
ELISA, direct and indirect: 1:5,000-1:10,000.
Optimal working dilutions must be determined by the end user.
ELISA, direct and indirect: 1:5,000-1:10,000.
Optimal working dilutions must be determined by the end user.
Linkage
Replaces: MABS176
Physical form
Format: Purified
Purified immunoglobulin in PBS, pH 7.4.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Adiponectin opposes endothelin-1-mediated vasoconstriction in the perfused rat hindlimb.
Bussey, CT; Kolka, CM; Rattigan, S; Richards, SM
American Journal of Physiology. Heart and Circulatory Physiology null
Jiung-Pang Huang et al.
Journal of molecular endocrinology, 60(4), 307-321 (2018-03-28)
Many studies have reported the causes of obese metabolic syndrome (MS); however, the causes of nonobese MS (NMS) remain unknown. In this study, we demonstrated that inflamed dysfunctional adipose tissue plays a crucial role in cholesterol-induced NMS. Control (C), high
Ruben Garcia-Martin et al.
Cell reports, 38(3), 110277-110277 (2022-01-20)
Exosomes/small extracellular vesicles (sEVs) can serve as multifactorial mediators of cell-to-cell communication through their miRNA and protein cargo. Quantitative proteomic analysis of five cell lines representing metabolically important tissues reveals that each cell type has a unique sEV proteome. While
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