F6428
Free Glycerol Reagent
used for quantitative enzymatic determination of glycerol
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
NACRES:
NA.25
Recommended Products
Quality Level
form
solid
usage
40 mL sufficient for 50 reactions
color
white to off-white
functional group
hydroxyl
relevant disease(s)
obesity
storage temp.
2-8°C
SMILES string
C(C(CO)O)O
InChI
1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
InChI key
PEDCQBHIVMGVHV-UHFFFAOYSA-N
General description
The Free Glycerol Reagent provides a precise method for measuring free endogenous glycerol levels through coupled enzyme reactions, bypassing the need for initial lipase hydrolysis. Unlike triglycerides, which are typically bound to proteins and transported in plasma, free glycerol circulates independently. The innovative approach involves enzymatic or alkaline hydrolysis of triglycerides, liberating glycerol and free fatty acids for accurate measurement.
The Serum Triglyceride Determination Kit (Catalog Number TR0100) offers a comprehensive solution for assessing glycerol, true triglycerides, or total triglycerides in serum or plasma samples. Utilizing lipase enzymatic hydrolysis, triglycerides are converted into glycerol and free fatty acids, enabling precise quantification through coupled enzyme reactions by F6428.
Unlike many commercially available triglyceride reagents, this method distinguishes between endogenous glycerol and glycerol derived from lipase hydrolysis of glycerides. With this advanced technology, researchers can confidently analyze glycerol levels with exceptional accuracy and reliability.
The Serum Triglyceride Determination Kit (Catalog Number TR0100) offers a comprehensive solution for assessing glycerol, true triglycerides, or total triglycerides in serum or plasma samples. Utilizing lipase enzymatic hydrolysis, triglycerides are converted into glycerol and free fatty acids, enabling precise quantification through coupled enzyme reactions by F6428.
Unlike many commercially available triglyceride reagents, this method distinguishes between endogenous glycerol and glycerol derived from lipase hydrolysis of glycerides. With this advanced technology, researchers can confidently analyze glycerol levels with exceptional accuracy and reliability.
Application
The Free Glycerol Reagent facilitates the quantitative enzymatic determination of glycerol levels in serum or plasma. Additionally, it may also be used to measure glycerol release from lipolysis in adipose tissue.
Biochem/physiol Actions
Glycerol undergoes phosphorylation through the enzymatic action of glycerol kinase (GK), utilizing adenosine-5′-triphosphate (ATP) to produce glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP). Subsequently, glycerol-1-phosphate (G-1-P) is oxidized by glycerol phosphate oxidase (GPO) to yield dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). This process involves peroxidase (POD), which catalyzes the coupling of hydrogen peroxide (H2O2) with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to generate a quinoneimine dye exhibiting an absorbance maximum at 540 nm.The observed increase in absorbance at 540 nm correlates directly with the concentration of free glycerol present in the sample. This method provides a reliable means for quantifying free glycerol levels, enabling accurate assessment of biochemical samples.
Features and Benefits
- Simplified Process: Streamline your workflow with a single reagent, offering a quick 5-minute reaction time.
- User-Friendly Design: Eliminate the need for weighing or mixing multiple reagents, ensuring ease of use and efficiency.
Principle
The free glycerol reagent uses coupled enzyme reactions resulting in an increase in absorbance at 540 nm that is directly proportional to the glycerol concentration.
Linkage
In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XIV (Authorisation List)
CAS No.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Claire Regazzetti et al.
Diabetes, 58(1), 95-103 (2008-11-06)
Obesity is characterized by an overgrowth of adipose tissue that leads to the formation of hypoxic areas within this tissue. We investigated whether this phenomenon could be responsible for insulin resistance by studying the effect of hypoxia on the insulin
Floriana Rotondo et al.
PeerJ, 4, e2725-e2725 (2016-12-06)
White adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defense, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT
Geonho Kim et al.
Journal of insect physiology, 106(Pt 1), 13-19 (2017-05-20)
Acetic acid is a fermentation product of many microorganisms, including some that inhabit the food and guts of Drosophila. Here, we investigated the effect of dietary acetic acid on oviposition and larval performance of Drosophila. At all concentrations tested (0.34-3.4%)
E Matthew Morris et al.
The Journal of physiology, 595(14), 4909-4926 (2017-05-16)
Low intrinsic aerobic capacity is associated with increased all-cause and liver-related mortality in humans. Low intrinsic aerobic capacity in the low capacity runner (LCR) rat increases susceptibility to acute and chronic high-fat/high-sucrose diet-induced steatosis, without observed increases in liver inflammation.
H Y Tan et al.
The Journal of physiology, 594(24), 7309-7326 (2016-08-26)
Loss of function of the melanocortin 4 receptor (MC4R) results in hyperphagia, obesity and increased growth. Despite knowing that MC4Rs control food intake, we are yet to understand why defects in the function of the MC4R receptor contribute to rapid
Articles
The potential for the prevention and treatment of cardiovascular disease through increased dietary intake of omega-3 (w-3) fish oils is not a recent scientific discovery.
Lipid Induced Insulin Resistance
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service