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QBD10199

Sigma-Aldrich

dPEG®4-biotin acid

>95% (HPLC)

Synonym(s):

Biotin-PEG-acid, Biotin-PEG4-COOH, Biotin-PEG4-acid, Carboxy-PEG4-biotin, PEG4-biotin acid

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About This Item

Empirical Formula (Hill Notation):
C21H37N3O8S
Molecular Weight:
491.60
UNSPSC Code:
12352106
NACRES:
NA.22

Assay

>95% (HPLC)

form

solid or viscous liquid

reaction suitability

reaction type: Biotinylations
reaction type: Pegylations

polymer architecture

shape: linear
functionality: monofunctional

shipped in

ambient

storage temp.

−20°C

Features and Benefits

dPEG4 biotin acid consists of biotin and a propionic acid-functionalized tetraethylene glycol derivative. Numerous functional or reactive groups can modify the terminal acid moiety, including the acylating agents N-hydroxysuccinimide (NHS) and 2,3,5,6-tetrafluorophenol (TFP). Also, dPEG4 biotin acid reacts directly with primary amines using a suitable carbodiimide such as 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). The amphiphilic dPEG linker gives biotin excellent solubility in water and aqueous buffer, where biotin usually is poorly soluble. dPEG4 biotin acid offers significantly superior solubility and performance characteristics compared to the traditional, highly hydrophobic biotinylation reagent known as LC-biotin. Moreover, although dPEG4 biotin acid and LC-biotin have comparable linker lengths, dPEG4 biotin acid will not trigger the aggregation and precipitation of conjugated biomolecules, even at high levels of biotin incorporation on the biomolecule. In contrast, LC-biotin triggers the aggregation and precipitation of biomolecules with the incorporation of a few LC-biotin groups onto the biomolecule.

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Legal Information

Products Protected under U.S. Patent #s 7,888,536 & 8,637,711 and European Patent #s 1,594,440 & 2,750,681
dPEG is a registered trademark of Quanta BioDesign

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Alexander Kuzmin et al.
Bioconjugate chemistry, 21(11), 2076-2085 (2010-10-23)
The utility of catalyst-free azide-alkyne [3 + 2] cycloaddition for the immobilization of a variety of molecules onto a solid surface and microbeads was demonstrated. In this process, the surfaces are derivatized with aza-dibenzocyclooctyne (ADIBO) for the immobilization of azide-tagged

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