RAS GTPases are modified by SUMOylation.

RAS GTPases are modified by SUMOylation.

Oncotarget (2018-02-13)

Byeong Hyeok Choi, Changyan Chen, Mark Philips, Wei Dai

ABSTRACT

RAS proteins are GTPases that participate in multiple signal cascades, regulating crucial cellular processes including cell survival, proliferation, differentiation, and autophagy. Mutations or deregulated activities of RAS are frequently the driving force for oncogenic transformation and tumorigenesis. Given the important roles of the small ubiquitin-related modifier (SUMO) pathway in controlling the stability, activity, or subcellular localization of key cellular regulators, we investigated here whether RAS proteins are posttranslationally modified (

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

E1014

Millipore

Nucleasi^® Benzonase, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

P8340

Sigma-Aldrich

Cocktail di inibitori delle proteasi, for use with mammalian cell and tissue extracts, DMSO solution

L6876

Sigma-Aldrich

Lisozima, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

SML1052

Sigma-Aldrich

2-D08, ≥98% (HPLC)