Passa al contenuto
Merck
  • Fluorescent methods in the study of UV-induced changes in structural and functional state of human blood lymphocytes.

Fluorescent methods in the study of UV-induced changes in structural and functional state of human blood lymphocytes.

Bulletin of experimental biology and medicine (2012-11-01)
V G Artyukhov, O V Putintseva, V A Vdovina, M V Pashkov, D V Vasilenko
ABSTRACT

Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m(2) was studied by methods of laser flow cytofluorometry, indirect immunofluorescence, and fluorescent probes. Using a combination of these methods, we have showed that UV light in the specified doses induced changes in the surface phenotype of T cells: stimulation or suppression of the expression of antigen-recognizing receptor complex molecules (CD3, CD4, and CD8 markers) and their redistribution on the surface of immunocompetent cells (capping effect) with the formation of receptor clusters of various types.

MATERIALI
N° Catalogo
Marchio
Descrizione del prodotto

Sigma-Aldrich
8-Anilino-1-naphthalenesulfonic acid ammonium salt, for fluorescence, ≥97.0% (HPLC)
Sigma-Aldrich
8-Anilino-1-naphthalenesulfonic acid
Sigma-Aldrich
8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate, for fluorescence, ≥95.0% (T)
Sigma-Aldrich
8-Anilino-1-naphthalenesulfonic acid ammonium salt, technical, ≥90% (NT)
Sigma-Aldrich
8-Anilino-1-naphthalenesulfonic acid hemimagnesium salt hydrate, for fluorescence, ≥95% (TLC)