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Documenti fondamentali

807457

Supelco

TOYOPEARL® HW-55F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 45 μm particle size

Sinonimo/i:

TOYOPEARL® HW-55 Size Exclusion Media

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About This Item

Codice UNSPSC:
41115700

Descrizione

HW-55F

Stato

beads

Confezionamento

bottle of 500 mL

Parametri

3 bar max. pressure

tecniche

HPLC: suitable
LPLC: suitable

Matrice

hydroxylated methacrylic polymer

Gruppo funzionale matrice

hydroxylated methacrylate phase

Dimensione particelle

45 μm

Dimensione pori

1000-200,000 Da MW range

pH di lavoro

2-13

Tecnica di separazione

size exclusion (SEC)

Descrizione generale

Toyopearl HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all Toyopearl "-550" designated products.

Applicazioni

TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Specifiche

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Stato fisico

Shipped in 20% (v/v) ethanol.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

Toyopearl is a registered trademark of Tosoh Corporation

Pittogrammi

Flame
GHS02

Avvertenze

Danger

Indicazioni di pericolo

H225

Classi di pericolo

Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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Sigma-Aldrich

G10120

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Sigma-Aldrich

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M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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