796-0150

HPLC Analysis of Acidic Herbicides in Water on a Polymeric C18 Column after SPE using Supelclean^™ ENVI^™-Carb

application for SPE, application for HPLC

Proprietà

• tecniche: HPLC: suitable; solid phase extraction (SPE): suitable
• Parametri analitici: sample preparation: SPE (Solid Phase Extraction); sample/matrix: Fresh 1 L water samples, dechlorinated with sodium thiosulfate when necessary, at ambient temperature and pH.; SPE tube/cartridge: Supelclean ENVI-Carb, 250 mg/6 mL (57092); condition: 10 mL DI water at 20 mL/min; sample addition: 0.9 L water sample at 20 mL/min; drying: 10 min using clean nitrogen; washing: 10 mL DI water at 20 mL/min; elution: 10 mL 0.1% phosphoric acid in methylene chloride:acetonitrile (80:20) at 5 mL/min; column: polymeric-coated silica-based PAH specialty column, 20 cm × 3 mm I.D., 5 μm (Supelco equivalent, SUPELCOSIL LC-PAH, available upon request); mobile phase: gradient, [A]: 0.05% phosphoric acid in DI water; [B]: acetonitrile; flow rate: 0.5 mL/min; column temp.: 50 °C; detector: photodiode array- peak width: 0.053 min, sampling interval: 0.320 sec, monitor 210 nm & 225 nm; injection: 10 μL of extract (4-5 ppb each analyte in water)
• Compatibilità: application for HPLC; application for SPE
• applicazioni: environmental

Descrizione

Applicazioni

using Zymark AutoTrace Extraction WorkStation 1.20

Note legali

ENVI is a trademark of Sigma-Aldrich Co. LLC

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analita

Dinoseb
2,4-D, analytical standard
Picloram, BioReagent, suitable for plant cell culture
2,4,5-T, PESTANAL^®
Pentachlorophenol
Tetrachloroterephthalic acid
Dicamba