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Key Documents

ZRB1870

Sigma-Aldrich

Anti-TRAF6 Antibody, clone 1M14 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

rabbit

Livello qualitativo

Ricombinante

expressed in HEK 293 cells

Coniugato

unconjugated

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

1M14, recombinant monoclonal

Descrizione

1M14 Clone

Nome Commerciale

ZooMAb® learn more

Forma fisica

lyophilized

PM

calculated mol wt 59.57 kDa
observed mol wt ~58 kDa

Purificato mediante

using Protein A

Reattività contro le specie

rat, monkey, human

Confezionamento

antibody small pack of 25 μL

Caratteristiche più verdi

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Convalida avanzata

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

tecniche

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

Isotipo

IgG

Sequenza dell’epitopo

C-terminus

N° accesso ID proteina

N° accesso UniProt

Categoria alternativa più verde

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... TRAF6(7189)

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificità

Clone 1M14 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects TNF receptor-associated factor 6 (TRAF6). It targets an epitope within 17 amino acids from the C-terminal region.

Immunogeno

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human TNF receptor-associated factor 6 (TRAF6).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRAF6 in K562 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TRAF6 in COS-7, C6, HEK293, and U2OS cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound TRAF6 peptide with a KD of 5.7 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TRAF6 in HEK293 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TRAF6 in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descrizione del bersaglio

TNF receptor-associated factor 6 (UniProt: Q9Y4K3; also known as EC:2.3.2.27, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6) is encoded by the TRAF6 (also known as RNF85) gene (Gene ID: 7189) in human. TRAF6 is a RING-type E3 ligase that plays a vital role in immune signal transduction pathways. It is composed of an N-terminal RING domain followed by at least four zinc finger domains (ZF1-4), a central coiled-coil domain, and a C-terminal TRAF-C domain. It catalyzes lysine 63-linked polyubiquitination using the Ubc13/Uev1A complex as the E2. It participates in many protective responses in immune and non-immune cells. TRAF6 is detected in the nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Upon receptor activation, TRAF6 is recruited to upstream signal transduction complexes and activates downstream IkB kinase (IKK) or MAP kinases leading to the activation of NF-kB and AP-1 transcription factors. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Dainichi, T., et al. (2019). Front. Immunol. 10; 1107; Fu, T-M., et al. (2018). Proc. Natl. Acad. Sci. USA.115(8); 1783-1788).

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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