ZRB04823

Anti-phospho-GluR1 (Ser831) Antibody, clone N453, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinonimo/i: AMPA-selective glutamate receptor 1, GluA1, GluR-A, GluR-K1, Glutamate receptor 1, Glutamate receptor ionotropic-AMPA 1
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit (recombinant)
• Livello qualitativo: 200
• Ricombinante: expressed in HEK 293 cells
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: N453, recombinant monoclonal; monoclonal
• Nome Commerciale: ZooMAb^® learn more
• Forma fisica: lyophilized
• PM: calculated mol wt 101.51 kDa; observed mol wt ~110 kDa
• Reattività contro le specie: rat, human
• Reattività contro le specie (prevista in base all’omologia): chicken, mouse
• Confezionamento: antibody small pack of 25 μL
• Caratteristiche più verdi: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• tecniche: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100; western blot: 1:250-1:1,000
• Isotipo: IgG
• N° accesso UniProt: P42261
• Categoria alternativa più verde: , Aligned
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: phosphorylation (pSer831)
• Informazioni sul gene: human ... GRIA1;GLUH1;GLUR1(2890)

Descrizione

Descrizione generale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificità

Clone N453 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glutamate Receptor 1 (GluR1) phosphorylated on serine 831 (or serine 849 with signal peptide included).

Immunogeno

KLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of Human Glutamate receptor 1.

Applicazioni

Anti-phospho-GluR1 (Ser831), clone N453 ZooMAb, Cat. No. ZRB04823, is a recombinant Rabbit Monoclonal Antibody that specifically targets Glutamate receptor 1 and has been tested for use in Immunohistochemistry (Paraffin), Phosphatase Assay, and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-GluR1 (Ser831) in rat brain tissue sections.

Phosphatase Assay Analysis: A 1:250 dilution from a representative lot detected phospho-GluR1 (Ser831) in Rat brain microsomal preparations.

Descrizione del bersaglio

Glutamate receptor 1 (UniProt: P42261; also known as GluR-1, AMPA-selective glutamate receptor 1, GluR-A, GluR-K1, Glutamate receptor ionotropic-AMPA 1, GluA1) is encoded by the GRIA1 (also known as GLUH1, GLUR1) gene (Gene ID: 2890) in human. Four different AMPA receptors have been described and are designated as GluR1, GluR2, GluR3, and GluR4. These receptors mediate fast excitatory neurotransmission in the brain and are involved in multiple brain functions, including cognition, movement, learning, and memory. Each of the AMPA receptor subunit can exist in either ′flip′ or ′flop′ form and the efficacy of glutamate is believed to be higher at the ′flip′ form. These receptors contain four sites where an agonist can bind. The binding site is formed by the N-terminal tail and the extracellular loop between transmembrane domains three and four. Upon agonist binding, these two loops move towards each other, which opens the pore. GluR-1 is a multi-pass membrane protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off. It is widely expressed throughout the nervous system and can exist in homotetrameric or heterotetrameric form. Tetramers may be formed by the dimerization of dimers. GluR-1 has several phosphorylation sites on the intracellular carboxy terminus. Many of these sites play a role in receptor regulation and synaptic plasticity. Reversible phosphorylation a of GluR-1 at serine 831 and 845 is shown to play a critical role in bidirectional synaptic plasticity. Both these phosphorylations are important in long-term potentiation and long-term depression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, HK., et al. (2010). J. Physiol. 103(1); 479-489).

Stato fisico

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Ricostituzione

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Note legali

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1