U211150

DMT-2′O-Methyl-rU Phosphoramidite

configured for ÄKTA^® and OligoPilot^®

• Sinonimo/i: 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-uridine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], DMT-2′O-Methyl-rU amidite
• Formula empirica (notazione di Hill): C₄₀H₄₉N₄O₉P
• Numero CAS: 110764-79-9
• Peso molecolare: 760.81
• Numero MDL: MFCD00792626
• Codice UNSPSC: 41116105
• ID PubChem: 329827609
• NACRES: NA.51

Proprietà

• Origine biologica: non-animal source (no BSE/TSE risk)
• Livello qualitativo: 300
• Nome Commerciale: Proligo Reagents
• Saggio: ≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
• Stato: powder
• tecniche: oligo synthesis: suitable
• Impurezze: ≤0.1% single unspecified Impurity (reversed phase HPLC); ≤0.3% mU2 (reversed phase HPLC, Hydrolysate); ≤0.3% mU3 (reversed phase HPLC, DMT-rme); ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤1.0% mU1 (reversed phase HPLC, DMT-rUme-DMT); ≤3% residual Solvent content
• Colore: white to off-white
• λ: conforms (UV/VIS Identity)
• Compatibilità: conforms to structure for H-NMR; conforms to structure for LC-MS; configured for ÄKTA^® and OligoPilot^®
• Profilo del nucleoside: base: uridine; base protecting group: none; 2' protecting group: methyl; 5' protecting group: DMT; deprotection: fast/standard
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1N5C=CC(=O)NC5=O
• InChI: 1S/C40H49N4O9P/c1-27(2)44(28(3)4)54(51-25-11-23-41)53-36-34(52-38(37(36)49-7)43-24-22-35(45)42-39(43)46)26-50-40(29-12-9-8-10-13-29,30-14-18-32(47-5)19-15-30)31-16-20-33(48-6)21-17-31/h8-10,12-22,24,27-28,34,36-38H,11,25-26H2,1-7H3,(H,42,45,46)/t34-,36-,37-,38-,54?/m1/s1; UVUOJOLPNDCIHL-XKZJCBTISA-N

Descrizione

Descrizione generale

DMT-2′O-Methyl-rU Phosphoramidite belongs to the class of 2′O-Methyl RNA Phosphoramidites.

Applicazioni

2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rU Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.

Caratteristiche e vantaggi

Its key features include:

• High yield of crude oligonucleotides
• Compatible with DNA synthesis
• Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA (concentrated ammonia/ 40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
• Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation
• Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers)
• 2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry

Altre note

The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules

Note legali

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable