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SRP5160

Sigma-Aldrich

14-3-3 σ, untagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinonimo/i:

14-3-3 σ, SFN, YWHAS, stratifin

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About This Item

Numero CAS:
1173938-21-0
Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in E. coli

Saggio

≥70% (SDS-PAGE)

Stato

buffered aqueous glycerol solution

PM

~29 kDa

N° accesso NCBI

NM_006142

applicazioni

cell analysis

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... SFN(2810)

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SRP5159SRP5154SRP5156
14-3-3 σ, untagged human recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5160

14-3-3 σ, untagged human

14-3-3 σ , GST tagged human recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5159

14-3-3 σ , GST tagged human

14-3-3 ε, untagged human recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5154

14-3-3 ε, untagged human

14-3-3 ζ, untagged human recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5156

14-3-3 ζ, untagged human

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

human

biological source

human

biological source

human

biological source

human

mol wt

~29 kDa

mol wt

~51 kDa

mol wt

~30 kDa

mol wt

~29 kDa

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Descrizione generale

14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest. Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.

Stato fisico

Supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Nota sulla preparazione

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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    Certificati d'analisi (COA)

    Lot/Batch Number
    Q030-3

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    T A Chan et al.
    Nature, 401(6753), 616-620 (1999-10-19)
    14-3-3Sigma is a member of a family of proteins that regulate cellular activity by binding and sequestering phosphorylated proteins. It has been suggested that 14-3-3sigma promotes pre-mitotic cell-cycle arrest following DNA damage, and that its expression can be controlled by
    H Hermeking et al.
    Molecular cell, 1(1), 3-11 (1998-07-11)
    Exposure of colorectal cancer (CRC) cells to ionizing radiation results in a cell-cycle arrest in G1 and G2. The G1 arrest is due to p53-mediated induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1/SDI1, but the basis for the G2 arrest is

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