SRP2093
SV40 T-antigen from simian virus
recombinant, expressed in insect cells, ≥80% (SDS-PAGE)
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352202
NACRES:
NA.77
Prodotti consigliati
Origine biologica
simian virus
Ricombinante
expressed in insect cells
Saggio
≥80% (SDS-PAGE)
Forma fisica
frozen liquid
PM
~83.3 kDa
Confezionamento
pkg of 5 μg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles
Concentrazione
400 μg/mL
Colore
clear colorless
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
simian virus ... SV40gp6(1489531)
Azioni biochim/fisiol
The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.
The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them.
Stato fisico
Clear and colorless frozen liquid solution
Nota sulla preparazione
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Time-sensitive reversal of hyperplasia in transgenic mice expressing SV40 T antigen.
Ewald D
Science, 273, 1384-1386 (1996)
Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.
Dornreiter I
The Embo Journal, 11, 769-776 (1992)
Simian virus 40 large T antigen: the puzzle, the pieces, and the emerging picture.
E Fanning
Journal of virology, 66(3), 1289-1293 (1992-03-01)
H H Chao et al.
Molecular and cellular biology, 20(20), 7624-7633 (2000-09-26)
At least three domains of simian virus 40 large T antigen (TAg) participate in cellular transformation. The LXCXE motif of TAg binds to all members of the retinoblastoma protein (pRB) family of tumor suppressors. The N-terminal 70 residues of TAg
Z Q Pan et al.
The Journal of biological chemistry, 271(36), 22111-22116 (1996-09-06)
The replication of simian virus 40 (SV40) DNA in vitro requires a trimeric single-stranded DNA (ssDNA)-binding protein called HSSB or RPA. HSSB supports the unwinding of DNA containing the SV40 origin in the presence of the viral-encoded T antigen and
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.