SRP0283
Sphingosine kinase 1 Active human
recombinant, expressed in baculovirus, ≥80% (SDS-PAGE)
Sinonimo/i:
SK1, SPHK1, SPK 1
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About This Item
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Ricombinante
expressed in baculovirus
Saggio
≥80% (SDS-PAGE)
Forma fisica
aqueous solution
PM
46.9 kDa
N° accesso NCBI
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... SPHK1(8877)
Descrizione generale
Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length with N-terminal His tag, MW = 46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Applicazioni
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Definizione di unità
One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min
Stato fisico
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Nota sulla preparazione
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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Deborah A Pasternack et al.
mBio, 6(5), e01291-e01215 (2015-10-08)
Sphingolipids are important constituents of cell membranes and also serve as mediators of cell signaling and cell recognition. Sphingolipid metabolites such as sphingosine-1-phosphate and ceramide regulate signaling cascades involved in cell proliferation and differentiation, autophagy, inflammation, and apoptosis. Little is
Sphingosine kinase 1 in viral infections
Carr, J., et al.
Reviews in Medical Virology, DOI: 10-DOI: 10 (2012)
Shi-Quan Liu et al.
International journal of colorectal disease, 27(12), 1569-1578 (2012-06-12)
Sphingosine kinase (SphK) 1 is an oncogenic enzyme promoting transformation, proliferation, and survival of a number of human tumor cells. However, its effect on colon cancer cell behavior has not been fully clarified. SphK1 plasmid or SphK1 shRNA transfection and
Jian Pan et al.
Journal of translational medicine, 9, 157-157 (2011-09-23)
Treatment failure for esophageal carcinoma is frequently due to lymph node metastasis and invasion to neighboring organs. The aim of the present study was to investigate invasion- and metastasis-related genes in esophageal carcinoma cells in vitro and in vivo. A
Francesca Bini et al.
Neuropharmacology, 63(4), 524-537 (2012-05-15)
1α,25-Dihydroxyvitamin D3 (1,25(OH)₂D₃), a crucial regulator of calcium/phosphorus homeostasis, has important physiological effects on growth and differentiation in a variety of malignant and non-malignant cells. Synthetic structural hormone analogues, with lower hypercalcemic side effects, are currently under clinical investigation. Sphingolipids
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