SRP0234

PI3 kinase [p110a(E545K)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)

• Sinonimo/i: PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110
• Codice UNSPSC: 12352200

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥55% (SDS-PAGE)
• Forma fisica: aqueous solution
• Attività specifica: ≥800 pmol/min-μg
• PM: 129 kDa; 88 kDa
• Confezionamento: pkg of 20 μg
• Concentrazione: 0.56 mg/mL
• N° accesso NCBI: U79143 (p110alpha); XM_043865 (p85alpha)
• N° accesso UniProt: P42336
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PIK3CA(5290)

Descrizione

Descrizione generale

Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definizione di unità

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1