SRP0146

PRMT5/MEP50 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Sinonimo/i: Arginine methyltransferase 5, HMT1 hnRNP methyltransferase-like 5(HRMT1L5), ICln-binding protein (IBP72) 72 kDa, Jak-binding protein 1 (JBP1), shk1 kinase-binding protein 1 homolog (SKB1)
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥70% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 73 kDa
• Confezionamento: pkg of 20 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_006109; NM_024102
• N° accesso UniProt: O14744
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PRMT5(10419)

Descrizione

Descrizione generale

Protein arginine methyltransferase 5 (PRMT5) also referred to as Janus kinase-binding protein 1, is encoded by the gene mapped to human chromosome 14q11.2. PRMT5 enzyme, containing 637 amino acids, belongs to type II enzymes, which is a subclass of PRMT family. PRMT5 is predominantly expressed at distinct level in various organs including heart, muscle and testis.
Human PRMT5 (GenBank Accession No. NM_006109), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 73kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Azioni biochim/fisiol

Protein arginine methyltransferase 5 (PRMT5) catalyzes the symmetric dimethylation of arginine residues. It is implicated in various biological functions such as ribosome biogenesis, assembly of the Golgi apparatus, cellular differentiation, cellular proliferation, apoptosis, and germ cell specification. Irregular expression of PRMT5 leads to several type of cancers such as leukemia and lymphoma, gastric cancer, breast cancer and colorectal cancer. PRMT5 plays an important role in the regulation of cell growth, proliferation and apoptosis in epithelial ovarian cancer cell lines A2780 and SKOV3. PRMT5 enables nuclear factor-κB (NF-κB) activation via demethylating arginine 30 (R30) on the p65 subunit of NF-κB. PRMT5 participates in transcriptional regulation by methylating histones H2A, H3R8, and H4R3 as well as by inhibiting the expression of suppressor of tumorigenicity 7 (ST7), and nonmetastatic 23 (NM23) genes. PRMT5 enhances DNA damage response via methylation of tumor suppressor p53 on R333, R335, and R337 in the oligomerization domain.

Definizione di unità

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable