SRP0133

EZH2/EED inactive  human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Sinonimo/i: ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥70% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 51 kDa (EED); 86 kDa (EZH2)
• Confezionamento: pkg of 20 μg
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_003797 (EED); NM_004456
• N° accesso UniProt: Q15910
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... EZH2(2146)

Descrizione

Descrizione generale

Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW= 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa, co-expressed in baculovirus expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.