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SNC50

Sigma-Aldrich

Mission® Small RNA Purification Kit

1 sufficient for 50 preparations

Sinonimo/i:

microRNA Isolation Kit

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About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

Livello qualitativo

200

impiego

sufficient for 50 preparations

tecniche

DNA extraction: suitable

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Descrizione generale

Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Applicazioni

mirPremier® microRNA Isolation Kit has been used to:

  • extract total RNA containing miRNA from grape edible plant derived exosome-like nanoparticles (EPDEN).
  • isolate small RNA from S.sirkka and S. napiecek, and S. arctica.
  • extract miRNA, from frozen rat livers for miRNA analysis.

Caratteristiche e vantaggi

  • Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
  • Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Can extract high-purity miRNA with no detectable large RNA
  • No dangerous organic extractions are involved.

Note legali

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H302 + H332,H315,H319

Classi di pericolo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is

Articoli

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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