SEQR

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

• Sinonimo/i: WGA kit, Whole transcriptome amplification
• Codice UNSPSC: 12352200
• NACRES: NA.55

Proprietà

• Livello qualitativo: 200
• tecniche: whole genome amplification: suitable
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

The SeqPlex^™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Applicazioni

SeqPlex^™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Caratteristiche e vantaggi

• Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
• Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
• No need to fragment DNA before sequencing.
• Amplifies ds-cDNA in 8 hours or less.
• Compatible with all next generation sequencing platforms except Pacific Bioscience.

Altre note

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Note legali

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable