SAB5801022

Alpaca Anti-Mouse IgG1(Fcγ) HRP single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Sinonimo/i: Recombinant Alpaca single variable domain secondary Nanobody
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: alpaca
• Ricombinante: expressed in E. coli
• Coniugato: horse radish peroxidase (HRP)
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: secondary antibodies
• Clone: recombinant monoclonal
• Forma fisica: lyophilized solid
• PM: 14 kDa
• Reattività contro le specie: mouse, Alpaca
• tecniche: ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• Misura: 0.1 mL
• Colore: colorless
• Isotipo: VHH
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Alpaca Anti-Mouse IgG1(Fcγ) HRP single domain Antibody is designed to specifically detect the Fc region of mouse IgG1. It is a monovalent, single domain, recombinant antibody to mouse IgG1(Fcγ fragment specific) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgG1(Fcγ) HRP single domain Antibody detects the Fc region of mouse IgG1 selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.


Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificità

Mouse IgG1(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogeno

Recombinant Alpaca single variable domain secondary nano antibody

Confezionamento

100 μg in Glass bottle

Stato fisico

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Ricostituzione

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Stoccaggio e stabilità

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

Altre note

It has weak cross reaction with mouse IgG3. No cross-reactivity with mouse IgG2b/2c, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317 - H412
• Consigli di prudenza: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Classi di pericolo: Aquatic Chronic 3 - Skin Sens. 1
• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable