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SAB4600042

Sigma-Aldrich

Anti-Mouse IgG (H+L), CF 488A antibody produced in goat

~2 mg/mL, affinity isolated antibody

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Livello qualitativo

Coniugato

CF 488A conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

mouse

Concentrazione

~2 mg/mL

tecniche

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 1-10 μg/mL

Fluorescenza

λex 490 nm; λem 515 nm

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.

Specificità

Binds all mouse IgGs

Immunogeno

mouse IgG (H+L)

Applicazioni

Anti-Mouse IgG (H+L), CF 488A antibody produced in goat has been used in immunohistochemistry and flow cytometry .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Azioni biochim/fisiol

IgGs,are glycoprotein antibodies that modulate several immune responses. It plays a major role in providing immunity to the infant from the mother via the placenta. IgG activates the complement system and regulates the antibody-dependent cell-mediated cytotoxicity (ADCC).

Caratteristiche e vantaggi

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Stato fisico

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Nota sulla preparazione

Protect from light.

Note legali

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2


Certificati d'analisi (COA)

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M Adamczyk et al.
Journal of immunological methods, 237(1-2), 95-104 (2000-03-22)
On-line liquid chromatography-electrospray ionization mass spectrometry (LC/ESI-MS) has been utilized to monitor the papain digestion of mouse monoclonal antibodies (mAb) of different subclasses (IgG1, IgG2a, IgG2b, and IgG3). The method is simple, rapid, sensitive, and allows for simultaneous determination of
Esam H Alhamad et al.
Annals of thoracic medicine, 10(4), 279-283 (2015-12-15)
Current protocols for detection of circulating fibrocytes (CFs) in peripheral blood described in various pulmonary and nonpulmonary disorders involve complex and time consuming, non standardized techniques. Testing a method to rapidly detect and quantify CFs using whole blood lysis flow
Patricia Palmeira et al.
Clinical & developmental immunology, 2012, 985646-985646 (2012-01-12)
Placental transfer of maternal IgG antibodies to the fetus is an important mechanism that provides protection to the infant while his/her humoral response is inefficient. IgG is the only antibody class that significantly crosses the human placenta. This crossing is
Rapid detection of circulating fibrocytes by flowcytometry in idiopathic pulmonary fibrosis
Alhamad E H, et al.
Annals of Thoracic Medicine, 10(4), 279-279 (2015)
Mechanical loading induces the expression of a Pol I regulon at the onset of skeletal muscle hypertrophy.
von Walden, F., et al.
American Journal of Physiology. Cell Physiology, 10, 1523-1530 (2012)

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