SAB4200845

Anti-Fibronectin antibody, Mouse monoclonal

clone IST-3, purified from hybridoma cell culture

• Sinonimo/i: Mouse Anti-FN
• Codice UNSPSC: 12352203

Proprietà

• Forma dell’anticorpo: purified from hybridoma cell culture
• Livello qualitativo: 200
• Clone: IST-3
• Forma fisica: liquid
• Reattività contro le specie: sheep, chicken, human, bovine, goat, dog, rabbit
• Concentrazione: ~1 mg/mL
• tecniche: immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells
• Isotipo: IgG1
• N° accesso UniProt: P02751
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Two types of Fibronectin are present in vertebrates: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin (formerly known as cold-insoluble globulin or CIG) is synthesized by hepatocytes, secreted to blood and upon tissue injury, is incorporated into fibrin clots effecting platelet function and mediating hemostasis.8 Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes. It is assembled by cells as they migrate into the clot to reconstitute damaged tissue.

Specificità

Monoclonal Anti-Fibronectin antibody specifically recognizes epitope located within the fourth type-three repeat of human plasma fibronectin. The antibody localizes plasma and cellular fibronectin both in its natural and denatured-reduced forms. The product reacts with fibronectin from human, goat, bovine4, porcine5, mouse6, rat7, dog and chicken origin.

Applicazioni

The antibody may be used in various immunochemical techniques including Immunoblotting1, ELISA, RIA1-2, Immunofluorescence3 and Immunohistochemistry4.

Azioni biochim/fisiol

Fibronectin (FN) is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides with molecular weights of 220-240 KDa. It is a ubiquitous and essential component of the extracellular matrix (ECM) which plays a vital role during tissue repair. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN gene. Fibronectin is suggested to enhance cell adhesion and spreading and to affect the routes of cell migration both in vivo and in culture.11 It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.

Stato fisico

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Stoccaggio e stabilità

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable