SAB4200767

Anti-Cathepsin D antibody, Mouse monoclonal

clone CTD-19, purified from hybridoma cell culture

• Sinonimo/i: Anti-CTSD, cleaved into the following 2 chains: Cathepsin D light chain and Cathepsin D heavy chain
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 300
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: CTD-19, monoclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: human, rabbit
• Concentrazione: ~1.0 mg/mL
• tecniche: immunoblotting: 2-4 μg/mL using human breast cancer MCF7 cell line; immunofluorescence: 5-10 μg/mL using HeLa cells; immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections
• Isotipo: IgG2a
• N° accesso UniProt: P07339
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CTSD(1509)

Descrizione

Descrizione generale

Anti-Cathepsin D antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the CTD-19 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with cathepsin D purified from human liver. Cathepsin D (CatD or CTSD) is synthesized in the rough endoplasmic reticulum as pre-proprotein. After removal of signal peptide, the pro-CatD is targeted to endosomes to form an active, ~48 kDa, single-chain intermediate then to the lysosomes to form the fully active mature protease, composed of a ~30 kDa heavy chain and a ~14 kDa light chain.

Immunogeno

Cathepsin D Purified from human liver

Applicazioni

Anti-Cathepsin D antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunohistochemistry
• immunofluorescence

Azioni biochim/fisiol

CatD plays numerous physiological functions in the cells including metabolic degradation of intracellular proteins and the activation of enzymatic precursors. In the central nervous system, CatD is particularly important for the control of neuronal homeostasis, cell migration and interneuron communication. CatD-mediated proteolysis mediates the degradation of unfolded/oxidized protein aggregates in lysosome. The level of CatD synthesized by the cells is increased in response to mitogenic signals from estrogen, epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin like growth factor-I (IGF- I). The ability of tumor cells to invade the extracellular matrix has been attributed to cathepsins released by tumor cells or associated with its plasma membrane.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable