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RAB0397

Sigma-Aldrich

Human PDGF-BB ELISA Kit

for serum, plasma, cell culture supernatant and urine

Sinonimo/i:

PDGF subunit B, PDGF-2, Platelet-derived growth factor subunit B

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About This Item

Codice UNSPSC:
41116158
NACRES:
NA.32

Reattività contro le specie

human

Confezionamento

kit of 96 wells (12 strips x 8 wells)

tecniche

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.549-400 pg/mL

Metodo di rivelazione

colorimetric

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... PDGFB(5155)

Descrizione generale

The Human PDGF-BB ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF-BB in serum, plasma, cell culture supernatants and urine.

Immunogeno

Recombinant Human PDGF-BB

Applicazioni

For research use only. Not for use in diagnostic procedures.
Human PDGF-BB ELISA Kit has been used to measure PDGF-BB (platelet-derived growth factor BB) concentration in human coronary artery smooth muscle cells (HCASMCs).

Azioni biochim/fisiol

PDGF-BB (platelet-derived growth factor BB) stimulates lymphatic metastasis and intratumoral lymphangiogenesis. PDGF-BB might be implicated in regulation of angiogenesis and endothelial proliferation. PDGF-BB expressed in placental endothelial cell activates signal transducer and activator of transcription 3 (STAT3) pathway and recruits human placental multipotent mesenchymal stromal cells (hPMSCs) for placental vascularization. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pittogrammi

Corrosion
GHS05

Avvertenze

Warning

Indicazioni di pericolo

H290

Consigli di prudenza

P234 - P390

Classi di pericolo

Met. Corr. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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PDGF-BB induces intratumoral lymphangiogenesis and promotes lymphatic metastasis.
Cao R
Cancer Cell, 6(4), 333-345 (2004)
PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.
Battegay EJ
The Journal of Cell Biology, 125(4), 917-928 (1994)
Callie Frey et al.
Platelets, 31(7), 952-959 (2020-01-15)
Patients with heart failure (HF) and left ventricular assist devices (LVAD) have dysregulated thrombo-inflammatory responses, mediated in part by platelets. While studies of platelet activation have been undertaken in HF, changes in the platelet transcriptome in HF patients following mechanical
Role of Myoendothelial Gap Junctions in the Regulation of Human Coronary Artery Smooth Muscle Cell Differentiation by Laminar Shear Stress.
Zhang Z
Cellular Physiology and Biochemistry, 39(2), 423-437 (2016)
Human placenta-derived multipotent mesenchymal stromal cells involved in placental angiogenesis via the PDGF-BB and STAT3 pathways.
Chen CY, et al.
Biology of Reproduction, 93, 103-103 (2015)
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