R506-05A

Rat Lung Fibroblasts: RLF, adult

• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• Origine biologica: rat lung
• Confezionamento: pkg of 500,000 cells
• Produttore/marchio commerciale: Cell Applications, Inc
• Modalità di accrescimento: Adherent
• Morfologia: fibroblast
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: asthma; tuberculosis; cancer; cardiovascular diseases
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

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RLF from Cell Applications, Inc. have been used to demonstrate that exaggerated (compared to adults) NF-κB activation in neonates in response to hyperoxia is mediated by phosphorylation of IκBα on Tyr42 (Wright, 2009). They were also used to elucidate melatonin effects and signaling pathway (Ben Soussia, 2012) and served as a control in studies investigating epithelial-mesenchimal transition as the source of myofibroblasts responsible for idiopathic pulmonary fibrosis (Willis, 2005; Jain, 2007).

Origine della linea cellulare

Lungs

Applicazioni

Pulmonary function, gene expression, healing & repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development.

Componenti

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Qualità

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Nota sulla preparazione

• 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 10 population doublings

Mantenimento delle subcolture

Please refer to the RLF Culture Protocol.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable