R4154

Anti-RbAp46, N-terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinonimo/i: Anti-RBBP7, Anti-Retinoblastoma-binding Protein 7
• Numero MDL: MFCD08705514
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: IgG fraction of antiserum
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 46 kDa
• Reattività contro le specie: human
• tecniche: indirect immunofluorescence: 1:200-1:400 using human HeLa cells fixed with paraformaldehyde/Triton; western blot: 1:1,000-1:2,000 μg/mL using nuclear extracts of human HeLa cells
• N° accesso UniProt: Q16576
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RBBP7(5931)

Descrizione

Descrizione generale

Retinoblastoma-associated binding protein 46 (RbAp46), also referred to as retinoblastoma-binding protein 7 (RBBP7) has 89% amino acid identity with RbAp48. This nuclear protein belongs to the WD-repeat protein family. It has four repeats that ends with Trp-Asp(WD) residues. RbAp46 is an important constituent of the histone modifying and remodeling complexes. RbAp46 gene is located on human chromosome Xp22.13

Specificità

Anti-RbAp46, N-terminal specifically recognizes human RbAp46.

Immunogeno

synthetic peptide corresponding to amino acids 1-14 of human RbAp46, conjugated to KLH via a C-terminal added cysteine residue. The sequence differs in mice by one amino acid.

Applicazioni

Anti-RbAp46, N-terminal antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Azioni biochim/fisiol

Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in the modification and remodeling of chromatin during cell growth and differentiation. It is associated with the nucleosomal remodeling and deacetylation complex (NURD). It may also play a key role in histone deacetylation and transcription repression.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)