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R1656

Sigma-Aldrich

RAF1 (EE) (306-end), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinonimo/i:

CMD1NN, NS5, RAF proto-oncogene serine/threonine-protein kinase, cRaf

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Origine biologica

fermentation/recombinant

Livello qualitativo

200

Ricombinante

expressed in baculovirus infected Sf9 cells

Nome Commerciale

PRECISIO® Kinase

Saggio

≥70% (SDS-PAGE)

Forma fisica

buffered aqueous glycerol solution

Attività specifica

5100-6900 nmol/min·mg

PM

~63 kDa

Produttore/marchio commerciale

Precision

tecniche

cell based assay: suitable

Solubilità

water: soluble

N° accesso ID proteina

NM 002880

N° accesso UniProt

P04049

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... RAF1(5894)

Descrizione generale

Research area: Cell Signaling

Rapidly Accelerated Fibrosarcoma (RAF) belongs to receptor tyrosine kinase (RTK) family. RAF exists as three isoforms A, B and C-RAF or RAF-1. RAF1 links Ras family members with mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling cascade. The RAF-1 gene is mapped to the human chromosome at 3p25.2.

Azioni biochim/fisiol

Rapidly Accelerated Fibrosarcoma 1(RAF1), a member of MAP kinase kinase kinase (MAP3K) family plays a vital role in cell signaling by directly interacting with GTPases and functions downstream in their signaling pathway. RaF1 is activated by Ras in the presence of growth-promoting signals, leading to the activation of mitogen-activated protein kinase kinase (MEK1/MEK2) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/ERK2) downstream. The activated ERK proteins control gene expression related to cell division, apoptosis, cell differentiation, and cell migration. The gene is mutated or upregulated in prostate and bladder cancer. Also, mutations are associated with Noonan syndrome, which leads to various physical abnormalities like craniofacial dysmorphisms, ocular, and musculoskeletal irregularities, neurocognitive impairment, and cardiac malformations.

Stato fisico

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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Benjamin R Nixon et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(1), 1138-1150 (2018-08-15)
Raf1/c-Raf is a well-characterized serine/threonine-protein kinase that links Ras family members with the MAPK/ERK signaling cascade. We have identified a novel splice isoform of human Raf1 that causes protein truncation and loss of the C-terminal kinase domain (Raf1-tr). We found
U R Rapp et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(14), 4218-4222 (1983-07-01)
We have molecularly cloned a unique acutely transforming replication-defective mouse type C virus (3611-MSV) and characterized its acquired oncogene. The viral genome closely resembles Moloney (M) murine leukemia virus (MuLV), except for a substitution in M-MuLV in the middle of
Helen J Blair et al.
BMC biology, 9, 14-14 (2011-03-02)
Evc is essential for Indian Hedgehog (Hh) signalling in the cartilage growth plate. The gene encoding Evc2 is in close proximity in divergent orientation to Evc and mutations in both human genes lead to the chondrodysplasia Ellis-van Creveld syndrome. Bioinformatic
Raf-1: a kinase currently without a cause but not lacking in effects.
P Li et al.
Cell, 64(3), 479-482 (1991-02-08)

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