QPBCA
QuantiPro™ BCA Assay Kit
for 0.5-30 μg/ml protein
Sinonimo/i:
BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prodotti consigliati
Categorie correlate
Applicazioni
In addition to protein determination and quantification in solution, the QuantiPro™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.
Caratteristiche e vantaggi
- Accurate across a broad range of protein concentrations
- High sensitivity; linear response form 0.5 to 30 μg/ml of protein
- Stable color complex
- Reduced susceptibility to detergents
Principio
Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.
Stoccaggio e stabilità
Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.
Note legali
QuantiPro is a trademark of Sigma-Aldrich Co. LLC
Solo come componenti del kit
N° Catalogo
Descrizione
- Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS
Applicazioni
N° Catalogo
Descrizione
Determinazione del prezzo
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Eneida V Reis et al.
Parasitology international, 63(1), 120-126 (2013-10-24)
Vaults are ribonucleoproteins (13 MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The
A new strategy for battling bacterial resistance: Turning potent, non-selective and potentially non-resistance-inducing biocides into selective ones
Ghanbar S, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine (2018)
Jingwei Zhang et al.
Acta biomaterialia, 10(7), 3254-3263 (2014-04-01)
The microporosity of calcium phosphate (CaP) ceramics has been shown to have an essential role in osteoinduction by CaP ceramics after ectopic implantation. Here we show that it is not the microporosity but the size of surface microstructural features that
PPAR-? agonist GL516 reduces oxidative stress and apoptosis occurrence in a rat astrocyte cell line
Neurochemistry International (2019)
Sook Hyun Chung et al.
Scientific reports, 6, 28993-28993 (2016-07-05)
Vascular changes and photoreceptor degeneration are features of age-related macular degeneration, diabetic retinopathy and macular telangiectasis. We have profiled the differential expression of microRNAs and analysed their target genes in transgenic mice in which induced Müller cell disruption results in
Articoli
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
Contenuto correlato
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.