P7623

PKD2 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), aqueous glycerol solution

• Sinonimo/i: Protein Kinase D2
• Numero MDL: MFCD07371065
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Ricombinante: expressed in E. coli
• Livello qualitativo: 200
• Saggio: ≥70% (SDS-PAGE)
• Stato: aqueous glycerol solution
• Attività specifica: 100 units/mg protein (Bradford)
• PM: protein apparent mol wt ~140 kDa
• N° accesso UniProt: Q9BZL6
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PRKD2(25865)

Descrizione

Applicazioni

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBTIDE substrate peptide (KRREILSRRPSYR), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P] ATP.

Azioni biochim/fisiol

PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase that contains two cysteine-rich motifs at the N terminus, a pleckstrin homology domain, and a catalytic domain. The PKD family of enzymes have been implicated in very diverse cellular functions, including Golgi organization and plasma membrane directed transport, metastasis, immune responses, apoptosis and cell proliferation.

Definizione di unità

One unit will incorporate one nanomole of phosphate into the CREBTIDE substrate (KRREILSRRPSYR) per minute at 30 °C at pH 7.2 using a final concentration of 50 μM [32P] ATP.

Stato fisico

Solution of 50 μl in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids