P5511
Protein Kinase A from bovine heart
≥0.8 units/μg protein, lyophilized powder
Sinonimo/i:
Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.77
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
Attività specifica
≥0.8 units/μg protein
Composizione
Protein, ≥70%
Temperatura di conservazione
−20°C
Informazioni sul gene
cow ... PRKACA(282322) , PRKACB(282323)
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Descrizione generale
Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.
Applicazioni
Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.
Azioni biochim/fisiol
Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.
Confezionamento
Package size based on protein content
Definizione di unità
One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.
Stato fisico
Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.
Nota sulla preparazione
Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).
Risultati analitici
Protein determined by biuret.
Inibitore
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Itohy T, et al.
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