P302-05

Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• Origine biologica: Porcine pulmonary artery (Normal healthy)
• Livello qualitativo: 100
• Forma fisica: solid
• Confezionamento: pkg of 500,000 cells
• Produttore/marchio commerciale: Cell Applications, Inc
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 38
• Morfologia: Endothelial
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: diabetes; cardiovascular diseases
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

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PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

Origine della linea cellulare

Artery

Applicazioni

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

Componenti

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Nota sulla preparazione

• 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Mantenimento delle subcolture

Please refer to the PPAEC Culture Protocol.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable