P1274

Poli-L-lisina

suitable for cell culture, Mol wt 70,000-150,000 by viscosity

• Sinonimo/i: L-Lysine homopolymer hydrobromide
• Numero CAS: 25988-63-0
• Numero MDL: MFCD00237230
• Codice UNSPSC: 12352209
• ID PubChem: 24898180
• NACRES: NA.26

Proprietà

• product name: Poli-L-lisina, mol wt 70,000-150,000 by viscosity
• Forma fisica: solid
• Livello qualitativo: 300
• PM: 70,000-150,000 by viscosity
• tecniche: cell culture | mammalian: suitable
• Colore: white to off-white
• Temperatura di conservazione: −20°C
• Stringa SMILE: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1; WBSCNDJQPKSPII-KKUMJFAQSA-N

Descrizione

Applicazioni

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Poly-L-lysine hydrobromide is used for the following applications:

• Used for some experiments with REF-52 cells, for coating
• For electrophysiological experiments, cells were seeded on poly-L-lysine hydrobromide coated or uncoated cover-slips
• Cell culture of rat neurons
• Indirect binding of monoclonal antibody via poly-llysine

Azioni biochim/fisiol

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Componenti

La poli-L-lisina è un polimero aminoacidico con carica positiva con circa un HBr per residuo di lisina. Il bromidrato consente alla poli-L-lisina di raggiungere la forma cristallina solubile in acqua. Una piccola quantità del prodotto può essere rinvenuta nella struttura beta perché il HBr interferisce con il legame idrogeno tra i gruppi amminici e i gruppi carbossilici o le frazioni che contengono N o O.

Avvertenza

Le soluzioni sterili sono stabili per un massimo di due anni se conservate a 2-8 °C. Il prodotto deve essere conservato essiccato a -20 °C.

Nota sulla preparazione

This product has a molecular weight of 70,000-150,000. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. None of the poly-L-lysine products have been exposed to trifluoroacetic acid and are dialyzed to remove any monomers, dimmers, or trimers, confirmed by thin layer chromatography. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm² of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Risultati analitici

Molecular weight based on viscosity and is also assayed by MALLS.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)