P0083

PRMT1 from rat

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous solution

• Sinonimo/i: HMT1-like 2, HRMT1L2, Heterogenious nuclear ribonucleoprotein methyltransferase 1-like 2, IR1B4, Interferon receptor 1-bound protein 4, Protein arginine N-methyl transferase 1
• Codice UNSPSC: 51111800
• NACRES: NA.32

Proprietà

• Ricombinante: expressed in E. coli
• Livello qualitativo: 200
• Saggio: ≥90% (SDS-PAGE)
• Stato: buffered aqueous solution
• N° accesso UniProt: Q63009
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: rat ... Prmt1(60421)

Descrizione

Azioni biochim/fisiol

Methyl transferases catalyze the addition of methyl groups to nitrogen, carbon, sulfur, and oxygen atoms of small molecules, lipids, proteins, and nucleic acids. Eight mammalian protein arginine methyltransferases (PRMT) have been identified. PRMT1 is the predominant member of the methyl transferases, which catalyzes the protein arginine N-methylation reactions. PRMT1 is implicated in various cellular processes including: transcription, RNA processing, and signal transduction.

Definizione di unità

The specific activity is ≥ 0.1 nmol/mgP/min. measured by ³H-AdoMet incorporation into histone for 30 minues at 30 °C.

Stato fisico

Solution of 50 mM Tris, pH 7.6, 5 mM DTT, 0.2% IGEPAL^® CA-630, 150 mM NaCl, and 30% glycerol (w/v).

Risultati analitici

The N-methyltransferase activity is determined by detecting the level of radiolabel transfer from ³H-AdoMet (Methyl donor) to histone (Cat. No. H4380), which is arginine rich (methyl acceptor).

Note legali

IGEPAL is a registered trademark of Solvay

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)