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Key Documents

MAK190

Sigma-Aldrich

ATP Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Sinonimo/i:

Adenosine triphosphate Assay Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Numero CE:
Codice UNSPSC:
12161503
NACRES:
NA.84

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

cancer; neurological disorders

Temperatura di conservazione

−20°C

Descrizione generale

Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes. Genetic disorders such as Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa affect the generation of ATP in the mitochondria.

Applicazioni

ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.

Compatibilità

Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.

Principio

The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.

Altre note

The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.

Solo come componenti del kit

N° Catalogo
Descrizione

  • ATP Assay Buffer

  • ATP Probe, in DMSO

  • ATP Converter

  • Developer Mix

  • ATP Standard, 1.0 μmole

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

188.6 °F

Punto d’infiammabilità (°C)

87 °C


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