MAK159
Mitochondrial Membrane Potential Kit
sufficient for 500 fluorometric tests (microplate readers)
Sinonimo/i:
JC-10 Assay, JC-10 Mitochondrial Membrane Potential Assay
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About This Item
Codice UNSPSC:
12161503
NACRES:
NA.25
Prodotti consigliati
impiego
sufficient for 500 fluorometric tests (microplate readers)
Metodo di rivelazione
fluorometric
Malattie correlate
cancer
Temperatura di conservazione
−20°C
Descrizione generale
Mitochondria generate a potential across their membranes due to the activities of enzymes of the electron transport chain. During apoptosis, collapse of the mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
Applicazioni
Mitochondrial Membrane Potential Kit has been used to measure mitochondrial membrane potential.
Compatibilità
This kit is suitable for the detection of Mitochondrial Membrane Potential in mammalian cells and for screening apoptosis inhibitors and activators using microplate readers.
Principio
This kit utilizes JC-10, a superior alternative to JC-1, for determining the loss of the MMP in cells. Although JC-1 is widely used in many labs, its poor water solubility often results in precipitation in aqueous buffers when used at higher concentrations. At higher concentrations, JC-10 exhibits greater aqueous solubility than JC-1. Similar to JC-1, JC-10 is a cationic, lipophilic dye that is concentrated and forms reversible red-fluorescent JC-10 aggregates (λex = 540/λem = 590 nm) in the mitochondria of cells with a polarized mitochondrial membrane. In apoptotic cells, MMP collapse results in the failure to retain JC-10 in the mitochondria and a return of the dye to its monomeric, green fluorescent form (λex = 490/λem = 525 nm). This kit can be used for monitoring apoptosis and for screening apoptosis inhibitors and activators.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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